[HTML][HTML] α-Thrombin-mediated phosphatidylinositol 3-kinase activation through release of Gβγ dimers from Gαq and Gαi2
R Goel, PJ Phillips-Mason, A Gardner… - Journal of Biological …, 2004 - ASBMB
Chinese hamster embryonic fibroblasts (IIC9 cells) express the Gα subunits Gα s, Gα i2, Gα
i3, Gα o, Gα q/11, and Gα 13. Consistent with reports in other cell types, α-thrombin
stimulates a subset of the expressed G proteins in IIC9 cells, namely G i2, G 13, and G q as
measured by an in vitro membrane [35 S] guanosine 5′-O-(3-thio) triphosphate binding
assay. Using specific Gα peptides, which block coupling of G-protein receptors to selective G
proteins, as well as dominant negative xanthine nucleotide-binding Gα mutants, we show …
i3, Gα o, Gα q/11, and Gα 13. Consistent with reports in other cell types, α-thrombin
stimulates a subset of the expressed G proteins in IIC9 cells, namely G i2, G 13, and G q as
measured by an in vitro membrane [35 S] guanosine 5′-O-(3-thio) triphosphate binding
assay. Using specific Gα peptides, which block coupling of G-protein receptors to selective G
proteins, as well as dominant negative xanthine nucleotide-binding Gα mutants, we show …