Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C

A Katz, D Wu, MI Simon - Nature, 1992 - nature.com
A Katz, D Wu, MI Simon
Nature, 1992nature.com
THE activation of heterotrimeric G proteins results in the exchange of GDP bound to the α-
subunit for GTP and the subsequent dissociation of a complex of the β-and γ-subunits (Gβγ).
The α-subunits of different G proteins interact with a variety of effectors1–7, but less is known
about the function of the free Gβγ complex. Gβγ has been implicated in the activation of a
cardiac potassium channel8, a retinal phospholipase A2 (ref. 9) and a specific receptor
kinase10, and in vitro reconstitution experiments indicate that the Gβγ complex can act with …
Abstract
THE activation of heterotrimeric G proteins results in the exchange of GDP bound to the α-subunit for GTP and the subsequent dissociation of a complex of the β- and γ-subunits (Gβγ). The α -subunits of different G proteins interact with a variety of effectors1–7, but less is known about the function of the free Gβγ complex. Gβγ has been implicated in the activation of a cardiac potassium channel8, a retinal phospholipase A2 (ref. 9) and a specific receptor kinase10, and in vitro reconstitution experiments indicate that the Gβγ complex can act with Gα subunit to modulate the activity of different isoforms of adenylyl cyclase11. Of two phospholipase activities that can be separated in extracts of HL-60 cells, purified Gβγ is found to activate one of them12. Here we report that in co-transfection assays Gβγ subunits specifically activate the β2 and not the β1 isoform of phospholipase, which acts on phosphatidylinositol. We use transfection assays to show also that receptor-mediated release of Gβγ from G proteins that are sensitive to pertussis toxin can result in activation of the phospholipase. This effect may be the basis of the pertussis-toxin-sensitive phospholipase C activation seen in some cell systems (reviewed in refs 13 and 14).
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