Activation of neutrophils by autocrine IL-17A–IL-17RC interactions during fungal infection is regulated by IL-6, IL-23, RORγt and dectin-2

PR Taylor, S Roy, SM Leal Jr, Y Sun, SJ Howell… - Nature …, 2014 - nature.com
PR Taylor, S Roy, SM Leal Jr, Y Sun, SJ Howell, BA Cobb, X Li, E Pearlman
Nature immunology, 2014nature.com
Here we identified a population of bone marrow neutrophils that constitutively expressed the
transcription factor RORγt and produced and responded to interleukin 17A (IL-17A (IL-17)).
IL-6, IL-23 and RORγt, but not T cells or natural killer (NK) cells, were required for IL-17
production in neutrophils. IL-6 and IL-23 induced expression of the receptors IL-17RC and
dectin-2 on neutrophils, and IL-17RC expression was augmented by activation of dectin-2.
Autocrine activity of IL-17A and its receptor induced the production of reactive oxygen …
Abstract
Here we identified a population of bone marrow neutrophils that constitutively expressed the transcription factor RORγt and produced and responded to interleukin 17A (IL-17A (IL-17)). IL-6, IL-23 and RORγt, but not T cells or natural killer (NK) cells, were required for IL-17 production in neutrophils. IL-6 and IL-23 induced expression of the receptors IL-17RC and dectin-2 on neutrophils, and IL-17RC expression was augmented by activation of dectin-2. Autocrine activity of IL-17A and its receptor induced the production of reactive oxygen species (ROS), and increased fungal killing in vitro and in a model of Aspergillus-induced keratitis. Human neutrophils also expressed RORγt and induced the expression of IL-17A, IL-17RC and dectin-2 following stimulation with IL-6 and IL-23. Our findings identify a population of human and mouse neutrophils with autocrine IL-17 activity that probably contribute to the etiology of microbial and inflammatory diseases.
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