Prevalence of polyreactive innate clones among graft-infiltrating B cells in human cardiac allograft vasculopathy
D Chatterjee, C Moore, B Gao, KJ Clerkin… - The Journal of Heart and …, 2018 - Elsevier
Background Cardiac allograft vasculopathy (CAV) has been associated with graft-infiltrating
B cells, although their characteristics are still unclear. In this study we examined the
frequency, localization and reactivity profile of graft-infiltrating B cells to determine their
contribution to the pathophysiology of CAV. Methods B cells, plasma cells and macrophages
were examined by immunohistochemistry in 56 allografts with CAV, 49 native failed hearts
and 25 autopsy specimens. A total of 102 B-cell clones were immortalized directly from the …
B cells, although their characteristics are still unclear. In this study we examined the
frequency, localization and reactivity profile of graft-infiltrating B cells to determine their
contribution to the pathophysiology of CAV. Methods B cells, plasma cells and macrophages
were examined by immunohistochemistry in 56 allografts with CAV, 49 native failed hearts
and 25 autopsy specimens. A total of 102 B-cell clones were immortalized directly from the …