Analysis of cell metabolism using LC-MS and isotope tracers
GM Mackay, L Zheng, NJF Van Den Broek… - Methods in …, 2015 - Elsevier
Here we discuss our methods to analyze small polar compounds involved in central carbon
metabolism using LC-MS. Methods described include sample extraction procedures for cells
and medium, as well as for plasma/serum, urine, CSF, and tissue samples. Different
extraction solvents are assessed. Our methods for using 13 C stable isotope tracers to
examine the kinetics and distributions of mass isotopologues of many metabolites are
discussed. Quantification methods are described for 13 C stable isotope tracer experiments …
metabolism using LC-MS. Methods described include sample extraction procedures for cells
and medium, as well as for plasma/serum, urine, CSF, and tissue samples. Different
extraction solvents are assessed. Our methods for using 13 C stable isotope tracers to
examine the kinetics and distributions of mass isotopologues of many metabolites are
discussed. Quantification methods are described for 13 C stable isotope tracer experiments …
