Cultured neonatal cardiomyocytes have long been used to study myofibrillogenesis and
myofibrillar functions. Cultured cardiomyocytes allow for easy investigation and
manipulation of biochemical pathways, and their effect on the biomechanical properties of
spontaneously beating cardiomyocytes. The following 2-day protocol describes the isolation
and culture of neonatal mouse cardiomyocytes. We show how to easily dissect hearts from
neonates, dissociate the cardiac tissue and enrich cardiomyocytes from the cardiac cell …