[HTML][HTML] Distinct roles of IL-7 and stem cell factor in the OP9-DL1 T-cell differentiation culture system
H Wang, LJ Pierce, GJ Spangrude - Experimental hematology, 2006 - Elsevier
OBJECTIVE: The OP9-DL1 culture system is an in vitro model for T-cell development in
which activation of the Notch pathway by Delta-like 1 promotes differentiation of mature T
cells from progenitors. The roles of specific cytokines in this culture system have not been
well defined, and controversy regarding the role of IL-7 has recently emerged. We examined
the roles played by IL-7, Flt3 ligand, and stem cell factor (SCF) in differentiation of adult bone
marrow cells in the OP9-DL1 culture system. METHODS: Hematopoietic progenitor cells …
which activation of the Notch pathway by Delta-like 1 promotes differentiation of mature T
cells from progenitors. The roles of specific cytokines in this culture system have not been
well defined, and controversy regarding the role of IL-7 has recently emerged. We examined
the roles played by IL-7, Flt3 ligand, and stem cell factor (SCF) in differentiation of adult bone
marrow cells in the OP9-DL1 culture system. METHODS: Hematopoietic progenitor cells …
