Increased cardiac output and preserved gas exchange despite decreased alveolar surface area in rats exposed to neonatal hyperoxia and adult hypoxia
KN Goss, RS Tepper, T Lahm… - American journal of …, 2015 - atsjournals.org
KN Goss, RS Tepper, T Lahm, SK Ahlfeld
American journal of respiratory cell and molecular biology, 2015•atsjournals.orgMaterials and Methods All procedures were approved by the Indiana University Animal Care
and Use Committee, as previously described (16, 17). Sprague-Dawley rat pups were
exposed to either room air (RA) or continuous hyperoxia (≥ 90% oxygen) for the first 10
postnatal days, corresponding to the period of saccular and early alveolar lung development
(18), and were then returned to RA. To understand how neonatal hyperoxic lung injury alters
the response to a secondary insult, from 10 to 12 weeks of age, animals were subsequently …
and Use Committee, as previously described (16, 17). Sprague-Dawley rat pups were
exposed to either room air (RA) or continuous hyperoxia (≥ 90% oxygen) for the first 10
postnatal days, corresponding to the period of saccular and early alveolar lung development
(18), and were then returned to RA. To understand how neonatal hyperoxic lung injury alters
the response to a secondary insult, from 10 to 12 weeks of age, animals were subsequently …
Materials and Methods
All procedures were approved by the Indiana University Animal Care and Use Committee, as previously described (16, 17). Sprague-Dawley rat pups were exposed to either room air (RA) or continuous hyperoxia (≥ 90% oxygen) for the first 10 postnatal days, corresponding to the period of saccular and early alveolar lung development (18), and were then returned to RA. To understand how neonatal hyperoxic lung injury alters the response to a secondary insult, from 10 to 12 weeks of age, animals were subsequently exposed to either RA or hypobaric hypoxia (H)(P atm= 362 mm Hg, equivalent to F i O 2 of 0.1 at sea level). At the end of the 2-week hypoxia exposure, cardiac output was assessed by echocardiography (VisualSonics Inc., Toronto, ON, Canada), followed by assessment of functional gas exchange by D f CO, as previously described (see Figure 1 for experimental timeline)(16, 17).
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