Isolation and differentiation of monocytes–macrophages from human blood
D Vijayan - Leucocytes: Methods and Protocols, 2012 - Springer
Leucocytes: Methods and Protocols, 2012•Springer
The prevalence of fungal infections remains high, and it is associated with significant
mortality and morbidity. Macrophages are heterogeneous population of effectors enriched in
regions of Candida colonization. These cells sense Candida, and are critical in the
resolution of these infections. Here, we describe how macrophages are generated in the
presence of colony-stimulating factor-1 (CSF-1); an important cytokine required for the
survival, proliferation and ex-vivo differentiation of monocytes to macrophages.
mortality and morbidity. Macrophages are heterogeneous population of effectors enriched in
regions of Candida colonization. These cells sense Candida, and are critical in the
resolution of these infections. Here, we describe how macrophages are generated in the
presence of colony-stimulating factor-1 (CSF-1); an important cytokine required for the
survival, proliferation and ex-vivo differentiation of monocytes to macrophages.
Abstract
The prevalence of fungal infections remains high, and it is associated with significant mortality and morbidity. Macrophages are heterogeneous population of effectors enriched in regions of Candida colonization. These cells sense Candida, and are critical in the resolution of these infections. Here, we describe how macrophages are generated in the presence of colony-stimulating factor-1 (CSF-1); an important cytokine required for the survival, proliferation and ex-vivo differentiation of monocytes to macrophages.
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