Expression of type 1 angiotensin II receptor subtypes and angiotensin II-induced calcium mobilization along the rat nephron.
N Bouby, A Hus-Citharel, J Marchetti… - Journal of the …, 1997 - journals.lww.com
The localization of two type 1 angiotensin II receptor subtype mRNA, AT1A and AT1B, was
determined by reverse transcription-PCR on microdissected glomeruli and nephron
segments. The coupling sensitivity of these two receptor subtypes was evaluated by
measuring variations in intracellular calcium ([Ca2+] i) elicited by angiotensin II (Ang II) in
structures expressing either AT1A or AT1B mRNA, using Fura-2 fluorescence. The highest
expression of AT1 mRNA was found in glomerulus, proximal tubule, and thick ascending …
determined by reverse transcription-PCR on microdissected glomeruli and nephron
segments. The coupling sensitivity of these two receptor subtypes was evaluated by
measuring variations in intracellular calcium ([Ca2+] i) elicited by angiotensin II (Ang II) in
structures expressing either AT1A or AT1B mRNA, using Fura-2 fluorescence. The highest
expression of AT1 mRNA was found in glomerulus, proximal tubule, and thick ascending …