[HTML][HTML] Cleavage of E-cadherin and β-catenin by calpain affects Wnt signaling and spheroid formation in suspension cultures of human pluripotent stem cells
SA Konze, L van Diepen, A Schröder, R Olmer… - Molecular & Cellular …, 2014 - ASBMB
The envisioned clinical and industrial use of human pluripotent stem cells and their
derivatives has given major momentum to the establishment of suspension culture protocols
that enable the mass production of cells. Understanding molecular changes accompanying
the transfer from adherent to suspension culture is of utmost importance because this
information can have a direct effect on the development of optimized culture conditions. In
this study we assessed the gene expression of human embryonic stem cells and induced …
derivatives has given major momentum to the establishment of suspension culture protocols
that enable the mass production of cells. Understanding molecular changes accompanying
the transfer from adherent to suspension culture is of utmost importance because this
information can have a direct effect on the development of optimized culture conditions. In
this study we assessed the gene expression of human embryonic stem cells and induced …