H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase
Nature communications, 2015•nature.com
DNA methylation is essential for mammalian development and physiology. Here we report
that the developmentally regulated H19 lncRNA binds to and inhibits S-
adenosylhomocysteine hydrolase (SAHH), the only mammalian enzyme capable of
hydrolysing S-adenosylhomocysteine (SAH). SAH is a potent feedback inhibitor of S-
adenosylmethionine (SAM)-dependent methyltransferases that methylate diverse cellular
components, including DNA, RNA, proteins, lipids and neurotransmitters. We show that H19 …
that the developmentally regulated H19 lncRNA binds to and inhibits S-
adenosylhomocysteine hydrolase (SAHH), the only mammalian enzyme capable of
hydrolysing S-adenosylhomocysteine (SAH). SAH is a potent feedback inhibitor of S-
adenosylmethionine (SAM)-dependent methyltransferases that methylate diverse cellular
components, including DNA, RNA, proteins, lipids and neurotransmitters. We show that H19 …
Abstract
DNA methylation is essential for mammalian development and physiology. Here we report that the developmentally regulated H19 lncRNA binds to and inhibits S-adenosylhomocysteine hydrolase (SAHH), the only mammalian enzyme capable of hydrolysing S-adenosylhomocysteine (SAH). SAH is a potent feedback inhibitor of S-adenosylmethionine (SAM)-dependent methyltransferases that methylate diverse cellular components, including DNA, RNA, proteins, lipids and neurotransmitters. We show that H19 knockdown activates SAHH, leading to increased DNMT3B-mediated methylation of an lncRNA-encoding gene Nctc1 within the Igf2-H19-Nctc1 locus. Genome-wide methylation profiling reveals methylation changes at numerous gene loci consistent with SAHH modulation by H19. Our results uncover an unanticipated regulatory circuit involving broad epigenetic alterations by a single abundantly expressed lncRNA that may underlie gene methylation dynamics of development and diseases and suggest that this mode of regulation may extend to other cellular components.
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