Microanalysis of non-heme iron in animal tissues
CJ Rebouche, CL Wilcox, JA Widness - Journal of biochemical and …, 2004 - Elsevier
The method recommended by the Iron Panel of the International Committee for the
Standardization in Haematology for measurement of serum iron was adapted for
measurement of non-heme iron in animal tissues. The method developed was designed
specifically to facilitate measurement of non-heme iron using as little as 10 mg of tissue, in a
final reaction volume of 60 μl. In this assay, tissue homogenates are treated with
hydrochloric acid and trichloroacetic acid and heated at 95° C. Non-heme iron is released …
Standardization in Haematology for measurement of serum iron was adapted for
measurement of non-heme iron in animal tissues. The method developed was designed
specifically to facilitate measurement of non-heme iron using as little as 10 mg of tissue, in a
final reaction volume of 60 μl. In this assay, tissue homogenates are treated with
hydrochloric acid and trichloroacetic acid and heated at 95° C. Non-heme iron is released …