Mast cell IL-6 improves survival from Klebsiella pneumonia and sepsis by enhancing neutrophil killing

RE Sutherland, JS Olsen, A McKinstry… - The Journal of …, 2008 - journals.aai.org
RE Sutherland, JS Olsen, A McKinstry, SA Villalta, PJ Wolters
The Journal of Immunology, 2008journals.aai.org
The pleiotropic cytokine IL-6 has favorable and harmful effects on survival from bacterial
infections. Although many innate immune cells produce IL-6, little is known about relevant
sources in vivo and the nature of its contributions to host responses to severe bacterial
infections. To examine these roles, we subjected mast cell-specific IL-6-deficient mice to the
cecal ligation and puncture model of septic peritonitis, finding that survival in these mice is
markedly worse than in controls. Following intranasal or ip inoculation with Klebsiella …
Abstract
The pleiotropic cytokine IL-6 has favorable and harmful effects on survival from bacterial infections. Although many innate immune cells produce IL-6, little is known about relevant sources in vivo and the nature of its contributions to host responses to severe bacterial infections. To examine these roles, we subjected mast cell-specific IL-6-deficient mice to the cecal ligation and puncture model of septic peritonitis, finding that survival in these mice is markedly worse than in controls. Following intranasal or ip inoculation with Klebsiella pneumoniae, IL-6−/− mice are less likely to survive than wild-type controls and at the time of death have higher numbers of bacteria but not inflammatory cells in lungs and peritoneum. Similarly, mast cell-specific IL-6-deficient mice have diminished survival and higher numbers of K. pneumoniae following ip infection. Neutrophils lacking IL-6 have greater numbers of live intracellular K. pneumonia, suggesting impaired intracellular killing contributes to reduced clearance in IL-6−/− mice. These results establish that mast cell IL-6 is a critical mediator of survival following K. pneumoniae infection and sepsis and suggest that IL-6 protects from death by augmenting neutrophil killing of bacteria.
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