[HTML][HTML] The phosphatidic acid–binding, polybasic domain is responsible for the differences in the phosphoregulation of lipins 1 and 3
S Boroda, S Takkellapati, RT Lawrence… - Journal of Biological …, 2017 - ASBMB
Lipins 1, 2, and 3 are Mg 2+-dependent phosphatidic acid phosphatases and catalyze the
penultimate step of triacylglycerol synthesis. We have previously investigated the
biochemistry of lipins 1 and 2 and shown that di-anionic phosphatidic acid (PA) augments
their activity and lipid binding and that lipin 1 activity is negatively regulated by
phosphorylation. In the present study, we show that phosphorylation does not affect the
catalytic activity of lipin 3 or its ability to associate with PA in vitro. The lipin proteins each …
penultimate step of triacylglycerol synthesis. We have previously investigated the
biochemistry of lipins 1 and 2 and shown that di-anionic phosphatidic acid (PA) augments
their activity and lipid binding and that lipin 1 activity is negatively regulated by
phosphorylation. In the present study, we show that phosphorylation does not affect the
catalytic activity of lipin 3 or its ability to associate with PA in vitro. The lipin proteins each …
