[HTML][HTML] Simultaneous imaging of GFP, CFP and collagen in tumors in vivousing multiphoton microscopy

E Sahai, J Wyckoff, U Philippar, JE Segall, F Gertler… - BMC …, 2005 - Springer
E Sahai, J Wyckoff, U Philippar, JE Segall, F Gertler, J Condeelis
BMC biotechnology, 2005Springer
Background The development of multiphoton laser scanning microscopy has greatly
facilitated the imaging of living tissues. However, the use of genetically encoded fluorescent
proteins to distinguish different cell types in living animals has not been described at single
cell resolution using multiphoton microscopy. Results Here we describe a method for the
simultaneous imaging, by multiphoton microscopy, of Green Fluorescent Protein, Cyan
Fluorescent Protein and collagen in vivo in living tumors. This novel method enables: 1) the …
Background
The development of multiphoton laser scanning microscopy has greatly facilitated the imaging of living tissues. However, the use of genetically encoded fluorescent proteins to distinguish different cell types in living animals has not been described at single cell resolution using multiphoton microscopy.
Results
Here we describe a method for the simultaneous imaging, by multiphoton microscopy, of Green Fluorescent Protein, Cyan Fluorescent Protein and collagen in vivo in living tumors. This novel method enables: 1) the simultaneous visualization of overall cell shape and sub-cellular structures such as the plasma membrane or proteins of interest in cells inside living animals, 2) direct comparison of the behavior of single cells from different cell lines in the same microenvironment in vivo.
Conclusion
Using this multi-fluor, multiphoton technique, we demonstrate that motility and metastatic differences between carcinoma cells of differing metastatic potential can be imaged in the same animal simultaneously at sub-cellular resolution.
Springer