Mouse islet of Langerhans isolation using a combination of purified collagenase and neutral protease
ND Stull, A Breite, R McCarthy, SA Tersey… - JoVE (Journal of …, 2012 - jove.com
The interrogation of beta cell gene expression and function in vitro has squarely shifted over
the years from the study of rodent tumorigenic cell lines to the study of isolated rodent islets.
Primary islets offer the distinct advantage that they more faithfully reflect the biology of
intracellular signaling pathways and secretory responses. Whereas the method of islet
isolation using tissue dissociating enzyme (TDE) preparations has been well established in
many laboratories1-4, variations in the consistency of islet yield and quality from any given …
the years from the study of rodent tumorigenic cell lines to the study of isolated rodent islets.
Primary islets offer the distinct advantage that they more faithfully reflect the biology of
intracellular signaling pathways and secretory responses. Whereas the method of islet
isolation using tissue dissociating enzyme (TDE) preparations has been well established in
many laboratories1-4, variations in the consistency of islet yield and quality from any given …