c-MET expression level in primary colon cancer: a predictor of tumor invasion and lymph node metastases
H Takeuchi, A Bilchik, S Saha, R Turner, D Wiese… - Clinical Cancer …, 2003 - AACR
H Takeuchi, A Bilchik, S Saha, R Turner, D Wiese, M Tanaka, C Kuo, HJ Wang, DSB Hoon
Clinical Cancer Research, 2003•AACRPurpose: Both c-MET and vascular endothelial growth factor (VEGF)-C expression are
important factors in primary carcinoma progression. We hypothesized that overexpression of
c-MET and/or VEGF-C mRNA in primary colorectal cancer (CRC) can predict tumor invasion
and regional metastasis. Experimental Design: The level of c-MET and VEGF-C mRNA
expression was assessed using a quantitative RT-RealTime PCR assay on early stage
primary CRC tumors (n= 36). Results: The c-MET mRNA copy number ranged from 1.18× …
important factors in primary carcinoma progression. We hypothesized that overexpression of
c-MET and/or VEGF-C mRNA in primary colorectal cancer (CRC) can predict tumor invasion
and regional metastasis. Experimental Design: The level of c-MET and VEGF-C mRNA
expression was assessed using a quantitative RT-RealTime PCR assay on early stage
primary CRC tumors (n= 36). Results: The c-MET mRNA copy number ranged from 1.18× …
Abstract
Purpose: Both c-MET and vascular endothelial growth factor (VEGF)-C expression are important factors in primary carcinoma progression. We hypothesized that overexpression of c-MET and/or VEGF-C mRNA in primary colorectal cancer (CRC) can predict tumor invasion and regional metastasis.
Experimental Design: The level of c-MET and VEGF-C mRNA expression was assessed using a quantitative RT-RealTime PCR assay on early stage primary CRC tumors (n = 36).
Results: The c-MET mRNA copy number ranged from 1.18 × 102 to 1.11 × 106 copies (median 5.17 × 104) per 250 ng of RNA from CRC specimens. c-MET mRNA copies in CRC specimens was significantly higher than that from normal colon mucosal epithelium (P = 0.0001). c-MET mRNA copies significantly correlated with the depth of invasion: T1 versus T2, P = 0.007; T1 versus T3/T4, P = 0.0001; T1 versus T2 versus T3/T4, P = 0.0005; and T1/T2 versus T3/T4, P = 0.011. c-MET copy number in primary CRC of N1/N2 staged patients was significantly higher than N0 cases (P < 0.03). Expression levels of c-MET mRNA were verified with immunohistochemistry analysis of c-MET protein expression in CRC specimens and normal mucosal epithelium. The VEGF-C mRNA copies of primary CRC assessed ranged from 0 to 1.65 × 105 copies (median 580). Although VEGF-C mRNA copies in CRC primary tumors were significantly higher than normal colon mucosal epithelium (P = 0.0008), it did not correlate with any major clinicopathological parameters of CRC.
Conclusions: This study indicates c-MET mRNA overexpression in primary CRC may be an important prognostic marker for early stage invasion and regional disease metastasis.
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