New bone formation and osteolysis by a metastatic, highly invasive canine prostate carcinoma xenograft
BE LeRoy, NK Thudi, MVP Nadella, RE Toribio… - The …, 2006 - Wiley Online Library
BE LeRoy, NK Thudi, MVP Nadella, RE Toribio, SH Tannehill‐Gregg, A Van Bokhoven…
The Prostate, 2006•Wiley Online LibraryBackground Osteoblastic metastases are commonly induced by prostate cancer. A canine
prostate carcinoma xenograft (Ace‐1) was developed and used to evaluate neoplastic
prostate cell growth, metastasis, and effects on bone formation in nude mice. Methods
Characteristics of the Ace‐1 cells were evaluated with histopathology, radiography, and
bioluminescent imaging (BLI). Immunohistochemistry and quantitative RT‐PCR were used
to evaluate the expression of factors important in the development of osteoblastic …
prostate carcinoma xenograft (Ace‐1) was developed and used to evaluate neoplastic
prostate cell growth, metastasis, and effects on bone formation in nude mice. Methods
Characteristics of the Ace‐1 cells were evaluated with histopathology, radiography, and
bioluminescent imaging (BLI). Immunohistochemistry and quantitative RT‐PCR were used
to evaluate the expression of factors important in the development of osteoblastic …
Background
Osteoblastic metastases are commonly induced by prostate cancer. A canine prostate carcinoma xenograft (Ace‐1) was developed and used to evaluate neoplastic prostate cell growth, metastasis, and effects on bone formation in nude mice.
Methods
Characteristics of the Ace‐1 cells were evaluated with histopathology, radiography, and bioluminescent imaging (BLI). Immunohistochemistry and quantitative RT‐PCR were used to evaluate the expression of factors important in the development of osteoblastic metastases.
Results
The Ace‐1 cells were invasive and induced bone formation and destruction. Radiographs demonstrated a mixed osteoblastic/osteolytic reaction. Lung and lymph node metastases occurred in 30% of mice. The tumor cells expressed parathyroid hormone‐related protein (PTHrP‐141 isoform), cathepsin K, keratins 8/18, and vimentin, but not keratins 5/14, and were androgen receptor negative. Intracardiac (IC) injections resulted in metastases in vertebrae and long bones.
Conclusions
The Ace‐1 xenograft is a useful model for investigating the pathogenesis of prostate cancer invasion and mixed osteoblastic/osteolytic bone metastases. Prostate © 2006 Wiley‐Liss, Inc.
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