Perforin and granzymes work in synergy to mediate cholangiocyte injury in experimental biliary atresia
P Shivakumar, R Mourya, JA Bezerra - Journal of hepatology, 2014 - Elsevier
Background & Aims Biliary atresia represents obstructive cholangiopathy in infants
progressing rapidly to cirrhosis and end-stage liver disease. Activated NK cells expressing
Nkg2d have been linked to bile duct injury and obstruction by establishing contact with
cholangiocytes. To define the mechanisms used by cytotoxic cells, we investigated the role
of perforin and granzymes in a neonatal mouse model of rotavirus (RRV)-induced biliary
atresia. Methods We used complementary cell lysis assays, flow cytometric analyses …
progressing rapidly to cirrhosis and end-stage liver disease. Activated NK cells expressing
Nkg2d have been linked to bile duct injury and obstruction by establishing contact with
cholangiocytes. To define the mechanisms used by cytotoxic cells, we investigated the role
of perforin and granzymes in a neonatal mouse model of rotavirus (RRV)-induced biliary
atresia. Methods We used complementary cell lysis assays, flow cytometric analyses …