[HTML][HTML] High throughput isolation and glycosylation analysis of IgG–variability and heritability of the IgG glycome in three isolated human populations

M Pučić, A Knežević, J Vidič, B Adamczyk… - Molecular & Cellular …, 2011 - ASBMB
All immunoglobulin G molecules carry N-glycans, which modulate their biological activity.
Changes in N-glycosylation of IgG associate with various diseases and affect the activity of
therapeutic antibodies and intravenous immunoglobulins. We have developed a novel 96-
well protein G monolithic plate and used it to rapidly isolate IgG from plasma of 2298
individuals from three isolated human populations. N-glycans were released by PNGase F,
labeled with 2-aminobenzamide and analyzed by hydrophilic interaction chromatography …