[CITATION][C] Cellular localization of cyclic nucleotide changes in rat superior cervical ganglion
MA Ariano, CA Briggs, DA McAfee - Cellular and Molecular Neurobiology, 1982 - Springer
MA Ariano, CA Briggs, DA McAfee
Cellular and Molecular Neurobiology, 1982•SpringerCyclic nucleotides were localized by immunohistochemistry using light microscopy in the rat
superior cervical ganglion. Cyclic GMP was found in the cytoplasm of postganglionic
neurons and their processes. Cyclic AMP immunoreactivity was intense in satellite cells
only. Ganglion sections incubated with antisera to cyclic AMP occasionally showed a faint
fluorescence in postganglionic neurons. 2. Cyclic AMP staining intensity was greatly
augmented within satellite cells following preganglionic stimulation or isoproterenol …
superior cervical ganglion. Cyclic GMP was found in the cytoplasm of postganglionic
neurons and their processes. Cyclic AMP immunoreactivity was intense in satellite cells
only. Ganglion sections incubated with antisera to cyclic AMP occasionally showed a faint
fluorescence in postganglionic neurons. 2. Cyclic AMP staining intensity was greatly
augmented within satellite cells following preganglionic stimulation or isoproterenol …
Summary
- 1. Cyclic nucleotides were localized by immunohistochemistry using light microscopy in the rat superior cervical ganglion. Cyclic GMP was found in the cytoplasm of postganglionic neurons and their processes. Cyclic AMP immunoreactivity was intense in satellite cells only. Ganglion sections incubated with antisera to cyclic AMP occasionally showed a faint fluorescence in postganglionic neurons.
- 2. Cyclic AMP staining intensity was greatly augmented within satellite cells following preganglionic stimulation or isoproterenol incubation. Stimulation of the postganglionic nerve did not alter cyclic AMP immunofluorescence.
- 3. Cyclic GMP immunofluorescence in the postganglionic neurons was increased by stimulation of the preganglionic nerve or following exposure to sodium azide. Stimulation of the postganglionic nerve increased cyclic GMP immunofluorescence in a few instances; the increases were small and were consistent with radioimmunoassay data.
- 4. There was no difference between experimental and control ganglia in the localization of cyclic nucleotide by cell type. Some treatments were selective for a cyclic nucleotide, but there was no apparent selectivity for cell type.
- 5. Qualitative changes in immunohistochemical localization of cyclic AMP and cyclic GMP reflect the quantitative alterations in these compounds assessed by radioimmunoassay after these same experimental procedures.
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