[HTML][HTML] Prominent role of platelets in the formation of circulating neutrophil-red cell heterocellular aggregates in sickle cell anemia

VM Dominical, L Samsel, JS Nichols, FF Costa… - …, 2014 - ncbi.nlm.nih.gov
VM Dominical, L Samsel, JS Nichols, FF Costa, JP McCoy Jr, N Conran, GJ Kato
Haematologica, 2014ncbi.nlm.nih.gov
High leukocyte counts are often observed in sickle cell anemia (SCA) and are associated
with increased clinical severity. In vivo animal model studies indicate that the adhesion of
leukocytes, particularly neutrophils, to the vessel wall may promote subsequent secondary
red blood cell (RBC) recruitment, in turn triggering the initiation of the vaso-occlusive
process. 1-3 While circulating neutrophilplatelet aggregates are well documented in
inflammatory conditions such as sepsis, atherosclerosis and SCA, 4-6 in which they are …
High leukocyte counts are often observed in sickle cell anemia (SCA) and are associated with increased clinical severity. In vivo animal model studies indicate that the adhesion of leukocytes, particularly neutrophils, to the vessel wall may promote subsequent secondary red blood cell (RBC) recruitment, in turn triggering the initiation of the vaso-occlusive process. 1-3 While circulating neutrophilplatelet aggregates are well documented in inflammatory conditions such as sepsis, atherosclerosis and SCA, 4-6 in which they are thought to augment tissue injury, 1, 6 the formation of leukocyte-RBC aggregates in humans has not been well studied. Circulating RBC-mononuclear cell aggregates have been reported in sickle cell disease (SCD), while the capture of SCD RBC by adherent leukocytes has been observed in vitro. 7-9 However, the existence of circulating neutrophil-RBC aggregates has, to our knowledge, yet to be described in humans, even in inflammatory settings. A total of 28 SCA patients in steady state, receiving HU (SCAHU; 15-30 mg/kg/day) or not, were recruited to the study (Table 1). Healthy controls (HbAA) were age-and haematologica 2014; 99: e214
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