Analysis of the mechanisms of action of the Saccharomyces cerevisiae dominant lethal cdc42 G12V and dominant negative cdc42 D118A mutations
CR Davis, TJ Richman, SB Deliduka… - Journal of Biological …, 1998 - ASBMB
The Saccharomyces cerevisiae Cdc42p GTPase is localized to the plasma membrane and
involved in signal transduction mechanisms controlling cell polarity. The mechanisms of
action of the dominant negative cdc42 D118A mutant and the lethal, gain of functioncdc42
G12V mutant were examined. Cdc42 D118A, C188S p and its guanine-nucleotide exchange
factor Cdc24p displayed a temperature-dependent interaction in the two-hybrid system,
which correlated with the temperature dependence of the cdc42 D118A phenotype and …
involved in signal transduction mechanisms controlling cell polarity. The mechanisms of
action of the dominant negative cdc42 D118A mutant and the lethal, gain of functioncdc42
G12V mutant were examined. Cdc42 D118A, C188S p and its guanine-nucleotide exchange
factor Cdc24p displayed a temperature-dependent interaction in the two-hybrid system,
which correlated with the temperature dependence of the cdc42 D118A phenotype and …
