Regulatory vs. inflammatory cytokine T-cell responses to mutated insulin peptides in healthy and type 1 diabetic subjects

M Nakayama, K McDaniel… - Proceedings of the …, 2015 - National Acad Sciences
M Nakayama, K McDaniel, L Fitzgerald-Miller, C Kiekhaefer, JK Snell-Bergeon…
Proceedings of the National Academy of Sciences, 2015National Acad Sciences
Certain class II MHC (MHCII) alleles in mice and humans confer risk for or protection from
type 1 diabetes (T1D). Insulin is a major autoantigen in T1D, but how its peptides are
presented to CD4 T cells by MHCII risk alleles has been controversial. In the mouse model
of T1D, CD4 T cells respond to insulin B-chain peptide (B: 9–23) mimotopes engineered to
bind the mouse MHCII molecule, IAg7, in an unfavorable position or register. Because of the
similarities between IAg7 and human HLA-DQ T1D risk alleles, we examined control and …
Certain class II MHC (MHCII) alleles in mice and humans confer risk for or protection from type 1 diabetes (T1D). Insulin is a major autoantigen in T1D, but how its peptides are presented to CD4 T cells by MHCII risk alleles has been controversial. In the mouse model of T1D, CD4 T cells respond to insulin B-chain peptide (B:9–23) mimotopes engineered to bind the mouse MHCII molecule, IAg7, in an unfavorable position or register. Because of the similarities between IAg7 and human HLA-DQ T1D risk alleles, we examined control and T1D subjects with these risk alleles for CD4 T-cell responses to the same natural B:9–23 peptide and mimotopes. A high proportion of new-onset T1D subjects mounted an inflammatory IFN-γ response much more frequently to one of the mimotope peptides than to the natural peptide. Surprisingly, the control subjects bearing an HLA-DQ risk allele also did. However, these control subjects, especially those with only one HLA-DQ risk allele, very frequently made an IL-10 response, a cytokine associated with regulatory T cells. T1D subjects with established disease also responded to the mimotope rather than the natural B:9–23 peptide in proliferation assays and the proliferating cells were highly enriched in certain T-cell receptor sequences. Our results suggest that the risk of T1D may be related to how an HLA-DQ genotype determines the balance of T-cell inflammatory vs. regulatory responses to insulin, having important implications for the use and monitoring of insulin-specific therapies to prevent diabetes onset.
National Acad Sciences