In vitro antimicrobial activity and the mode of action of indole-3-carbinol against human pathogenic microorganisms
WS Sung, DG Lee - Biological and Pharmaceutical Bulletin, 2007 - jstage.jst.go.jp
WS Sung, DG Lee
Biological and Pharmaceutical Bulletin, 2007•jstage.jst.go.jpMATERIALS AND METHODS Materials Indole-3-carbinol (I3C), propionic acid, amphotericin
B, trehalase and RNase A were purchased from the Sigma Chemical Co.(St. Louis, MO,
USA). Stock solutions of I3C and amphotericin B were prepared in dimethyl sulfoxide
(DMSO), and solutions of propionic acid were prepared in sterile distilled water and stored at
20 C. For all the experiments, a final concentration of 1% DMSO was used as the solvent
carrier.
B, trehalase and RNase A were purchased from the Sigma Chemical Co.(St. Louis, MO,
USA). Stock solutions of I3C and amphotericin B were prepared in dimethyl sulfoxide
(DMSO), and solutions of propionic acid were prepared in sterile distilled water and stored at
20 C. For all the experiments, a final concentration of 1% DMSO was used as the solvent
carrier.
MATERIALS AND METHODS
Materials Indole-3-carbinol (I3C), propionic acid, amphotericin B, trehalase and RNase A were purchased from the Sigma Chemical Co.(St. Louis, MO, USA). Stock solutions of I3C and amphotericin B were prepared in dimethyl sulfoxide (DMSO), and solutions of propionic acid were prepared in sterile distilled water and stored at 20 C. For all the experiments, a final concentration of 1% DMSO was used as the solvent carrier.
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