[HTML][HTML] Glucose deprivation stimulates O-GlcNAc modification of proteins through up-regulation of O-linked N-acetylglucosaminyltransferase
O-Linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification of proteins
that functions as a nutrient sensing mechanism. Here we report on regulation of O-
GlcNAcylation over a broad range of glucose concentrations. We have discovered a
significant induction of O-GlcNAc modification of a limited number of proteins under
conditions of glucose deprivation. Beginning 12 h after treatment, glucose-deprived human
hepatocellular carcinoma (HepG2) cells demonstrate a 7.8-fold increase in total O-GlcNAc …
that functions as a nutrient sensing mechanism. Here we report on regulation of O-
GlcNAcylation over a broad range of glucose concentrations. We have discovered a
significant induction of O-GlcNAc modification of a limited number of proteins under
conditions of glucose deprivation. Beginning 12 h after treatment, glucose-deprived human
hepatocellular carcinoma (HepG2) cells demonstrate a 7.8-fold increase in total O-GlcNAc …