Extended and direct evaluation of RT‐Qu IC assays for Creutzfeldt‐Jakob disease diagnosis
BR Groveman, CD Orrú, AG Hughson… - Annals of Clinical …, 2017 - Wiley Online Library
Annals of Clinical and Translational Neurology, 2017•Wiley Online Library
Abstract Real‐Time Quaking‐Induced Conversion (RT‐Qu IC) testing of human
cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD
patients from those without prion disease. Here, using CSF samples from 113 CJD and 64
non‐prion disease patients, we provide the first direct and concurrent comparison of our
improved RT‐Qu IC assay to our previous assay, which is similar to those commonly used
internationally for CJD diagnosis. This extended comparison demonstrated a~ 21% increase …
cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD
patients from those without prion disease. Here, using CSF samples from 113 CJD and 64
non‐prion disease patients, we provide the first direct and concurrent comparison of our
improved RT‐Qu IC assay to our previous assay, which is similar to those commonly used
internationally for CJD diagnosis. This extended comparison demonstrated a~ 21% increase …
Abstract
Real‐Time Quaking‐Induced Conversion (RT‐QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non‐prion disease patients, we provide the first direct and concurrent comparison of our improved RT‐QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2‐day reduction in average detection time, and 100% specificity.
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