Measurement and analysis of extracellular acid production to determine glycolytic rate
SA Mookerjee, MD Brand - JoVE (Journal of Visualized Experiments), 2015 - jove.com
Extracellular measurement of oxygen consumption and acid production is a simple and
powerful way to monitor rates of respiration and glycolysis1. Both mitochondrial (respiration)
and non-mitochondrial (other redox) reactions consume oxygen, but these reactions can be
easily distinguished by chemical inhibition of mitochondrial respiration. However, while
mitochondrial oxygen consumption is an unambiguous and direct measurement of
respiration rate2, the same is not true for extracellular acid production and its relationship to …
powerful way to monitor rates of respiration and glycolysis1. Both mitochondrial (respiration)
and non-mitochondrial (other redox) reactions consume oxygen, but these reactions can be
easily distinguished by chemical inhibition of mitochondrial respiration. However, while
mitochondrial oxygen consumption is an unambiguous and direct measurement of
respiration rate2, the same is not true for extracellular acid production and its relationship to …
