Lipid extraction using a monophasic chloroform/methanol/water mixture, coupled with
functional group selective derivatization and direct infusion nano-ESI-high-
resolution/accurate MS, is shown to facilitate the simultaneous analysis of both highly polar
and nonpolar lipids from a single retina lipid extract, including low abundance highly polar
ganglioside lipids, nonpolar sphingolipids, and abundant glycerophospholipids. Quantitative
comparison showed that the monophasic lipid extraction method yielded similar lipid …