The discovery that the MPC1 and MPC2 genes encode the protein components of the
mitochondrial pyruvate carrier (MPC) has invigorated studies of mitochondrial pyruvate
transport and its regulation in normal and disease states. Indeed, recent reports have
demonstrated MPC involvement in the control of cell fate in cancer and gluconeogenesis in
models of type 2 diabetes. Biochemical measurements of MPC activity are foundational for
understanding the role of pyruvate transport in health and disease. We developed a 96-well …