[HTML][HTML] Analysis of M phase-specific phosphorylation of DNA topoisomerase II
K Kimura, N Nozaki, T Enomoto, M Tanaka… - Journal of Biological …, 1996 - ASBMB
In mammalian cells, two isoforms of DNA topoisomerase II (topo II), topo IIα and topo IIβ, are
phosphorylated. The phosphorylation of topo IIβ changes its apparent molecular mass
determined by SDS-polyacrylamide gel electrophoresis from 180 to 190 kDa in mitotic cells,
whereas topo IIα affects it only slightly (Kimura, K., Nozaki, N., Saijo, M., Kikuchi, A., Ui, M.,
and Enomoto, T.(1994) J. Biol. Chem. 269, 24523-24526). Here we examined the stability of
the protein and the phosphate moiety of each topo II isoform, as the cells progressed from M …
phosphorylated. The phosphorylation of topo IIβ changes its apparent molecular mass
determined by SDS-polyacrylamide gel electrophoresis from 180 to 190 kDa in mitotic cells,
whereas topo IIα affects it only slightly (Kimura, K., Nozaki, N., Saijo, M., Kikuchi, A., Ui, M.,
and Enomoto, T.(1994) J. Biol. Chem. 269, 24523-24526). Here we examined the stability of
the protein and the phosphate moiety of each topo II isoform, as the cells progressed from M …