[HTML][HTML] Binding of adeno-associated virus type 5 to 2, 3-linked sialic acid is required for gene transfer
RW Walters, SMP Yi, S Keshavjee, KE Brown… - Journal of Biological …, 2001 - ASBMB
Recombinant adeno-associated viruses (AAV) are promising gene therapy vectors.
Whereas AAV serotype 2-mediated gene transfer to muscle has partially replaced factor IX
deficiency in hemophilia patients, its ability to mediate gene transfer to the lungs for cystic
fibrosis is hindered by lack of apical receptors. However, AAV serotype 5 infects human
airway epithelia from the lumenal surface. We found that in contrast to AAV2, the apical
membrane of airway epithelia contains abundant high affinity receptors for AAV5. Binding …
Whereas AAV serotype 2-mediated gene transfer to muscle has partially replaced factor IX
deficiency in hemophilia patients, its ability to mediate gene transfer to the lungs for cystic
fibrosis is hindered by lack of apical receptors. However, AAV serotype 5 infects human
airway epithelia from the lumenal surface. We found that in contrast to AAV2, the apical
membrane of airway epithelia contains abundant high affinity receptors for AAV5. Binding …