[HTML][HTML] Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein
T Kamitani, K Kito, HP Nguyen, ETH Yeh - Journal of Biological Chemistry, 1997 - ASBMB
NEDD8 is a novel 81 amino acid polypeptide which is 60% identical and 80% homologous
to ubiquitin. Northern blot analysis showed that the NEDD8 message was developmentally
down-regulated. In adult tissues, NEDD8 expression was mostly restricted to the heart and
skeletal muscle. Antiserum specific for NEDD8 detected a 6-kDa monomer in SK-N-SH,
BJAB, and HL60 cell lysates. A 14-kDa band was also detected in BJAB, HL60, and SK-
MEL28 but not in SK-N-SH and K562 cell lysates. An approximately 90-kDa band was …
to ubiquitin. Northern blot analysis showed that the NEDD8 message was developmentally
down-regulated. In adult tissues, NEDD8 expression was mostly restricted to the heart and
skeletal muscle. Antiserum specific for NEDD8 detected a 6-kDa monomer in SK-N-SH,
BJAB, and HL60 cell lysates. A 14-kDa band was also detected in BJAB, HL60, and SK-
MEL28 but not in SK-N-SH and K562 cell lysates. An approximately 90-kDa band was …