The novel anti-CD19 chimeric antigen receptors with humanized scFv (single-chain variable fragment) trigger leukemia cell killing
L Qian, D Li, L Ma, T He, F Qi, J Shen, X Lu - Cellular immunology, 2016 - Elsevier
The molecular design of CARs (Chimeric Antigen Receptors), especially the scFv, has been
a major part to use of CAR-T cells for targeted adoptive immunotherapy. To address this
issue, we chose a vector backbone encoding a second-generation CAR based on efficacy of
a murine scFv-based CAR. Next, we generated a panel of humanized scFvs and tested in
vitro for their ability to direct CAR-T cells to specifically lyse, proliferate, and secrete
cytokines in response to antigen-bearing targets. Furthermore, in a xenograft model of …
a major part to use of CAR-T cells for targeted adoptive immunotherapy. To address this
issue, we chose a vector backbone encoding a second-generation CAR based on efficacy of
a murine scFv-based CAR. Next, we generated a panel of humanized scFvs and tested in
vitro for their ability to direct CAR-T cells to specifically lyse, proliferate, and secrete
cytokines in response to antigen-bearing targets. Furthermore, in a xenograft model of …