Wiebke Arlt, Katharina Lang, Alice J. Sitch, Anna S. Dietz, Yara Rhayem, Irina Bancos, Annette Feuchtinger, Vasileios Chortis, Lorna C. Gilligan, Philippe Ludwig, Anna Riester, Evelyn Asbach, Beverly A. Hughes, Donna M. O’Neil, Martin Bidlingmaier, Jeremy W. Tomlinson, Zaki K. Hassan-Smith, D. Aled Rees, Christian Adolf, Stefanie Hahner, Marcus Quinkler, Tanja Dekkers, Jaap Deinum, Michael Biehl, Brian G. Keevil, Cedric H.L. Shackleton, Jonathan J. Deeks, Axel K. Walch, Felix Beuschlein, Martin Reincke
Immunohistochemistry with digital image analysis for steroidogenic enzyme expression in aldosterone-producing adrenal adenoma tissue in relation to in vivo 24-hour glucocorticoid excretion.
A tissue microarray with adenoma tissue from 57 patients was studied by immunohistochemistry for expression of CYP11B1, required for cortisol and 11β-hydroxyandrostenedione synthesis, and CYP11B2, the enzyme responsible for aldosterone synthesis, followed by digital image analysis for quantification of staining intensity. Representative immunohistochemistry examples from 2 patients with in vivo glucocorticoid excretion within the lowest and highest quartiles, respectively, are shown (total original magnification in all panels, ×20).