Infusion of in vitro–derived T cell progenitor (proT) therapy with hematopoietic stem cell transplant aids the recovery of the thymus damaged by total body irradiation. To understand the interaction between proTs and the thymic microenvironment, WT mice were lethally irradiated and given T cell–deficient (
Michelle J. Smith, Dawn K. Reichenbach, Sarah L. Parker, Megan J. Riddle, Jason Mitchell, Kevin C. Osum, Mahmood Mohtashami, Heather E. Stefanski, Brian T. Fife, Avinash Bhandoola, Kristin A. Hogquist, Georg A. Holländer, Juan Carlos Zúñiga-Pflücker, Jakub Tolar, Bruce R. Blazar
T cell progenitors home to the thymus but migrate with reduced velocity on irradiated stroma.
Lethally irradiated B6 recipients were given congenically marked B6 BM with or without luciferase-positive progenitors (proTs). Animals were monitored using a Xenogen bioluminescent imager. Data are representative of 2 separate experiments, with 4–5 animals in each group. (