Distinct mucosal and systemic immunological characteristics in transgender women potentially relating to HIV acquisition

Transgender women (TGW) are disproportionally affected by HIV infection, with a global estimated prevalence of 19.9%, often attributed to behavioral risk factors, with less known about biological factors. We evaluated potential biological risk factors for HIV acquisition in TGW at the sites of viral entry by assessing immune parameters of the neovaginal surface and gut mucosa. The neovagina in TGW, compared with the vagina in cisgender women (CW), shows distinct cell composition and may pose a more inflammatory environment, evidenced by increased CD4+ T cell activation and higher levels of soluble markers of inflammation (C-reactive protein, soluble CD30). Increased inflammation may be driven by microbiome composition, as shown by a greater abundance of Prevotella and a higher Shannon Diversity Index. In addition, we have observed higher frequency of CD4+CCR5+ target cells and decreased DNA methylation of the CCR5 gene in the gut mucosa of TGW compared with CW and men who have sex with men, which was inversely correlated with testosterone levels. The rectal microbiome composition in TGW appears to favor a proinflammatory milieu as well as mucosal barrier disruption. Thus, it is possible that increased inflammation and higher frequencies of CCR5-expressing target cells at sites of mucosal viral entry may contribute to increased risk of HIV acquisition in TGW, with further validation in larger studies warranted.


Introduction
HIV has differential impacts on communities and key populations.In a large meta-analysis, the odds ratio for being infected with HIV in transgender women (TGW) compared with all adults of reproductive age was 48.8 (95% CI 21.2-76.3)irrespective of country income level, with a global pooled estimated prevalence of 19.9% (1,2).This statistic may be driven by several factors, including high rates of condom-less anal intercourse and sexually transmitted infections (STIs) and poorer access to HIV testing and preventive health care services due to increased stigma and legal discrimination.Risk is further increased by psychosocial health problems including mental health issues, substance use, and targeted violence (3,4).
However, there is little research addressing whether this may also be compounded by increased biological risk for HIV acquisition due to biological factors unique to TGW as part of the male-to-female transition.To minimize secondary sex characteristics generally associated with men, TGW undergo feminizing hormone treatment to increase female characteristics, which includes the usage of progestogens, estrogens, Transgender women (TGW) are disproportionally affected by HIV infection, with a global estimated prevalence of 19.9%, often attributed to behavioral risk factors, with less known about biological factors.We evaluated potential biological risk factors for HIV acquisition in TGW at the sites of viral entry by assessing immune parameters of the neovaginal surface and gut mucosa.The neovagina in TGW, compared with the vagina in cisgender women (CW), shows distinct cell composition and may pose a more inflammatory environment, evidenced by increased CD4 + T cell activation and higher levels of soluble markers of inflammation (C-reactive protein, soluble CD30).Increased inflammation may be driven by microbiome composition, as shown by a greater abundance of Prevotella and a higher Shannon Diversity Index.In addition, we have observed higher frequency of CD4 + CCR5 + target cells and decreased DNA methylation of the CCR5 gene in the gut mucosa of TGW compared with CW and men who have sex with men, which was inversely correlated with testosterone levels.The rectal microbiome composition in TGW appears to favor a proinflammatory milieu as well as mucosal barrier disruption.Thus, it is possible that increased inflammation and higher frequencies of CCR5-expressing target cells at sites of mucosal viral entry may contribute to increased risk of HIV acquisition in TGW, with further validation in larger studies warranted.

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JCI Insight 2023;8 (16):e169272 https://doi.org/10.1172/jci.insight.169272and antiandrogens (5).Recommended feminizing hormone regimens are composed of estrogen to promote female secondary sexual characteristics and androgen-lowering drugs to inhibit male secondary sexual characteristics by decreasing endogenous testosterone production or testosterone activity (6,7).However, in Thailand, there is high use of feminizing hormone treatment reported outside the reference regimen as these drugs can be purchased without a medical prescription (8).Progesterone and estrogen are known to have differential effects on mucosal epithelial barriers, modulating cellular expression of CCR5, the primary co-receptor for entry of R5 tropic HIV into CD4 + T cells (9), and α4β7, a cellular gut-homing marker (10), as well as on vaginal epithelial thickness (11,12).Sex hormone levels are also linked to inflammation, induced by the impact they have on the composition of microbial communities (13).In addition, multiple studies have demonstrated that the microbiome is a major determinant of the local immune environment, with sex hormones such as progesterone estradiol and testosterone directly impacting the microbiome composition in the gut (14,15).In general, cisgender men (CM) exhibit a lower microbial diversity than cisgender women (CW), with healthy CW showing predominantly Firmicutes and CM a higher abundance of Bacteroides Prevotella (16,17).Sex hormones directly regulate bacterial growth; e.g., Prevotella intermedius takes up estradiol and progesterone, favoring bacterial expansion and in turn affecting inflammation (18).Furthermore, the intestinal mucosa is known to be steroidogenic, and colonic epithelial cells express estrogen receptor β, making the intestinal mucosa likely to be affected by feminizing hormone treatment (19,20).
To further affirm gender identity, TGW may undergo a variety of surgical procedures such as use of injectable fillers, breast augmentation, and vaginoplasty (7).Those without affordable access to sterile liquid silicone may use substances such as oil or petroleum jelly as injectable fillers (7), which may predispose to systemic immune activation and render CD4 + T cells more susceptible to HIV infection.Gender affirmation surgery (GAS) by vaginoplasty aims to create a functional vagina which can be achieved by a variety of surgical techniques including penile skin inversion, colonic flap, or peritoneal flap vaginoplasty (21,22).Currently, penile skin inversion is the most widely used technique for GAS.The cell composition of the skin creates a nonlubricated neovagina due to lack of columnar epithelial cells that is prone to stenosis and requires regular dilatation and intensive longitudinal postsurgical care (7,23).It is therefore possible that this creates a more inflammatory milieu both due to mechanical abrasion and potentially substandard long-term care.In addition, neovaginas reconstructed by penile skin inversion have diverse polymicrobial communities that can increase immune activation and decrease epithelial barrier function (24).Taken together, it is plausible that these changes may predispose to a more favorable neovaginal environment for HIV transmission, given that cervicovaginal inflammation in CW predisposes to increased HIV acquisition (25,26); however, it is of note that HIV entry in the female genital tract (FGT) occurs mainly at the transition zone/cervix (27).
More detailed and systematic studies are therefore needed to better understand potential biological factors unique to TGW that could influence the risk of HIV acquisition.Here, we report evidence that the unique biology of TGW sets them apart from CW and men who have sex with men (MSM).This includes (i) a higher inflammatory environment in the neovagina reconstructed by penile skin inversion that may be driven by a highly diverse microbiome, (ii) an increase in CCR5 expression in the intestinal mucosa related to hormonal levels, and (iii) distinct rectal microbiome composition impacting gut mucosal homeostasis.
Differential composition of CD4 + and CD8 + T cell populations in vaginal mucosa compared with neovaginal surfaces grafted by penile skin inversion.Establishing the immune profile of CD4 + T cells, the primary target cells of HIV in the FGT, is critical to better understand the risk of HIV acquisition (28).Several studies focusing on characterizing the CD4 + T cell subsets in the vaginal mucosa have shown that vaginal CD4 + T cells express high levels of CCR5 and are rapidly depleted in HIV/SIV infection (29).However, there is little known about the cellular composition of the surfaces of neovaginas in TGW created through different vaginoplasties (21,24).To evaluate cell composition of the neovagina, grafted by penile skin inversion, we collected 2 sequential neovaginal and vaginal swabs, respectively, to identify and characterize different T cell subsets (Figure 1A).In the vaginal mucosa we observed a high frequency of CD4 + T cells (median 20.6%), and while the neovaginal surfaces also contained CD4 + T cells, they were observed in far lower frequencies (median 1.5%, P = 0.05; Figure 1B).Similarly, the vaginal mucosal contained a higher frequency of CD4 + T cells expressing the CCR5 HIV co-receptor compared with neovaginal surfaces (median: vaginal 43.1% vs. neovaginal 8.0%, P = 0.01; Figure 1E).Interestingly, we also observed a significantly lower frequency of CD4 + CCR5 + T cells in the peripheral blood of TGW (median 8.4%) compared with CW (median 18.6%, P = 0.003), while there were no differences in the frequency of CD4 + and CD8 + T cells and the CD4/CD8 ratio (Supplemental Figure 2, A, D, and H).Surprisingly, CD8 + T cells were highly enriched on neovaginal surfaces (median 66.7%) compared with vaginal mucosa (median 3.6%; P = 0.0003), resulting in a significantly decreased CD4/CD8 ratio (median: vaginal 2.5 vs. neovaginal 0.01; P = 0.0005; Figure 1, C and D).
Cellular and soluble inflammatory markers are distinct between the vaginal mucosa and the neovaginal surfaces.Similar to the importance of the CD4 + T cell immune profiles in the vaginal mucosa, several studies also have shown that cellular and soluble markers of inflammation in the vagina significantly contribute to the risk of HIV acquisition (25,30,31).However, those data are scarce in relation to the inflammatory profile of the neovagina, with only a few studies attempting to assess this in TGW after GAS (24).Consequently, we quantified levels of T cell activation by measuring the frequency of HLA-DR/CD38 coexpression and the frequency of cycling T cells by the expression of Ki67, in the vaginal mucosa and neovaginal surfaces, respectively, in CD4 + , CD4 + CCR5 + , and CD8 + T cells (Figure 1A).In CD4 + T cells the frequency of cycling cells was significantly increased in neovaginal surfaces compared with vaginal mucosa (median: neovaginal 28.0% vs. vaginal 1.8%; P = 0.02, Figure 2A).In contrast, CD4 + T cell activation, indicated by the coexpression of HLA-DR/CD38, was higher on vaginal compared with neovaginal CD4 + T cells (median: neovaginal 0.1% vs. vaginal 6.9%; P = 0.0003; Figure 2B).A similar trend was observed for CD4 + CCR5 + T cells, where a significantly higher activation status was observed in vaginal CD4 + CCR5 + T cells (median: neovaginal 0.1% vs. vaginal 12.8%; P = 0.0003; Figure 2D); however, there was no difference in the frequency of cycling CD4 + CCR5 + T cells (median: neovaginal 0.1% vs. vaginal 3.7%; P = NS; Figure 2C).A higher frequency of Ki67-expressing and programmed cell death 1-expressing (PD-1-expressing) neovaginal CD8 + T cells was observed compared with those found in vaginal mucosa (median Ki67: neovaginal 12.0% vs. vaginal 2.3%; P = 0.05; Figure 2E; median PD-1: neovaginal 20.2% vs. vaginal 5.5%; P = 0.002; Figure 2G).The frequency of activated CD8 + T cells was also higher in vaginal mucosa compared with neovaginal surfaces (median: neovaginal 0.1% vs. vaginal 14.7%; P = 0.01; Figure 2F).There was no difference observed in the frequency of activated and cycling CD4 + , CD4 + CCR5 + , and CD8 + T cells in peripheral blood in TGW and CW (Supplemental Figure 2).
Next, we explored the different levels of soluble inflammatory cytokines in vaginal and neovaginal secretions as elevated levels of inflammatory cytokines such as macrophage inflammatory protein 1β (MIP-1β), IP-10, and IL-8 have been previously linked to a higher risk of HIV acquisition in women (25).We observed that the levels of C-reactive protein (CRP) and soluble CD30 (sCD30) were significantly elevated in neovaginal secretions compared with vaginal secretions (median CRP: neovaginal 548 pg/mL vs. vaginal 480 pg/mL; P = 0.02; Figure 2H; median sCD30: neovaginal 2.45 pg/mL vs. vaginal 0.55 pg/mL; P = 0.04; Figure 2I).Other inflammatory cytokines tested that have previously been linked to a higher risk of HIV acquisition, including monocyte chemoattractant protein-1 (MCP-1), IP-10, MIP-1β, IL-8, TNFα-RI, and IFN-α2, were not different between vaginal and neovagina secretions (data not shown).In plasma there were no differences between biomarker levels in CW and TGW, except for MCP-1 plasma levels with a median of 0.26 pg/mL in TGW showing increased levels over a median 0.18 pg/mL in CW (P = 0.05; data not shown).
Distinct composition of vaginal and neovaginal microbial communities relate to local inflammatory profiles.Alterations of the mucosal environment in the FGT, such as inflammation and hormone levels, have been associated with an increased risk of HIV acquisition (24).The FGT microbiome has been closely associated with the inflammatory profile, with both the presence of specific taxa and their properties and activities being critical determinants of mucosal inflammation (32,33).Based on those observations, we assessed the vaginal and the neovaginal microbiome utilizing 16s rRNA gene sequencing of swab samples.The composition of the neovaginal microbiome was significantly different from the vaginal microbiome on the phylum level, as indicated by a higher median Shannon Diversity Index of 1.4 compared with 0.6, respectively (P = 0.002; Figure 3A).These distinct differences were also supported by principal component analysis, indicating distinct clustering of all neovaginal samples compared with the vaginal microbiome samples (Figure 3B).The relative abundance of Gardnerella vaginalis, a notable component of the vaginal microflora, was observed in 80% of the vaginal samples compared with 0% of neovaginal samples (Figure 3C).Moreover, a species of lactobacilli, Lactobaccilus iners, was abundant in only vaginal but not neovaginal samples (Figure 3C).Distinct to the neovaginal compartment, we observed the abundance of Finegoldia magna, an anaerobic, Gram-positive bacterium that colonizes the skin and has been linked to inflammation (Figure 3C) (34,35).We also observed a greater median Shannon Diversity Index on the species level in the neovaginal (2.2) compared with the vaginal microbiome (0.8, P = 0.0007, Figure 4A and Figure 3C).These findings support prior research showing that the microflora of the neovagina constitutes a mixed microflora of aerobe and anaerobe species usually found on the skin, in the intestinal microflora, or in a bacterial vaginosis microflora (35).We noted a high abundance of Prevotella

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JCI Insight 2023;8(16):e169272 https://doi.org/10.1172/jci.insight.169272 in the neovaginal microbiome (P = 0.02; Figure 4B), which has been shown to be part of nonoptimal microbial communities in the vaginal microbiome of CW as well as in dysbiosis of the gut microbiome linked to inflammation in both instances (36).In contrast, the vaginal microbiome was largely dominated by Lactobacillus with a significantly higher abundance compared with the neovaginal microbiome (P = 0.0003; Figure 4C).Interestingly, the Shannon Diversity Index on the species level for the neovaginal/vaginal microbiome directly correlated significantly with the soluble markers of inflammation CRP (r = 0.59, P = 0.006; Figure 4D) and sCD30 (r = 0.47, P = 0.04; Figure 4E) as well as with the frequency of cycling (Ki67-expressing) CD4 + T cells (r = 0.56, P = 0.03; Figure 4F) in the respective compartments.This observation is in line with several studies that have shown an impact of the vaginal microbiome diversity on local inflammatory profiles (30,37) and suggests a similar mechanism for the neovaginal microbiome, linking the higher bacterial diversity in the neovagina with higher local cellular and soluble inflammation.
Increased frequency of CD4 + CCR5 + T cells in the gut mucosa of TGW relates to changes in CCR5 DNA methylation.TGW in this study and other studies (38) have reported engagement in neovaginal and/or in anal intercourse (Table 1 and Supplemental Figure 1B).Previous research has shown that the hormonal milieu and hormone levels can impact vaginal HIV susceptibility, with progesterone impacting the epithelial thickness and increasing the expression of the HIV co-receptor CCR5 on CD4 + T cells (7).However, little is known about hormonal effects on the gut mucosa and how potential changes might impact HIV susceptibility during anal intercourse.Therefore, as a first step, we explored the frequency of CD4 + CCR5 + T cells in the gut mucosa of CW, TGW, and MSM.We observed that the frequency of CD4 + CCR5 + T cells in the sigmoid colon was significantly higher in CW (median 64.5%) and in TGW (median 70.0%) compared with MSM (median 53.0%, P = 0.004 and P = 0.006, respectively; Figure 5A).This observation was verified when determining the absolute number of CD4 + CCR5 + T cells per gram of tissue (median absolute numbers: MSM: 3.3 vs. CW: 4.7; P = 0.006 and MSM: 3.3 vs. TGW: 4.7; P = 0.029; Figure 5B).The epigenetic feature DNA methylation within cis-regulatory regions of the CCR5 gene has been shown to correlate with CCR5 levels on T cells (39).Therefore, we examined DNA methylation states at the CCR5 gene in the gut mucosa.We observed DNA at loci cg22066626 located within an annotated regulatory region of the CCR5 gene was significantly hypomethylated in TGW compared with MSM and similar trends for CW (P = 0.014 and P = 0.06, respectively; Figure 5C).The DNA methylation levels related to the CCR5 gene were inversely associated with the frequency of CD4 + CCR5 + T cells in the gut mucosa (r = -0.41,P = 0.03), supporting the differences seen in CCR5 expression in the gut despite the low number of participants (Figure 5D).Interestingly, the frequency of CD4 + CCR5 + T cells in the gut was inversely associated with the level of serum testosterone (P = -0.68,r = 0.001; Figure ) compared with the vagina (n = 8), while there is no difference in the frequency of cycling CD4 + CCR5 + (C) T cells observed between the vagina and the neovagina.In contrast, CD4 + (B), CD4 + CCR5 + (D), and CD8 + (F) T cells in the vagina had a higher activation status compared with the neovagina indicated by the coexpression of HLA-DR and CD38.However, there was also a significant increase in PD-1-expressing CD8 + T cells observed in the neovagina (G), which was not seen in CD4 + T cells (data not shown).The only soluble biomarkers out of 18 tested that were significantly different between the vagina and the neovagina were the inflammation makers CRP (median: neovaginal 548 pg/mL vs. vaginal 480 pg/mL) (H) and sCD30 (median: neovaginal 2.45 pg/mL vs. vaginal 0.55 pg/mL) (I) that were significantly increased in neovaginal secretions (H).Differences between groups were analyzed using unpaired t tests.

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JCI Insight 2023;8(16):e169272 https://doi.org/10.1172/jci.insight.1692725E), implying a potential impact of hormone levels on the expression of the HIV co-receptor CCR5 on CD4 + T cells.There was no correlation between estradiol E2 levels and the frequency of mucosal CD4 + CCR5 + T cells observed (P = 0.22, r = 0.33; data not shown).
Different rectal microbiome composition in TGW compared with MSM and CW suggests increased disruption of gut mucosal homeostasis and rectal inflammation.In addition to the assessment of mucosal cell populations that could contribute to the increased risk of HIV acquisition in TGW, we also determined 16 soluble biomarkers of inflammation, including markers indicative for microbial translocation (MTL) and enterocyte damage, such as Zonulin, sCD14, and intestinal fatty acid binding protein (I-FABP), in plasma and in rectal secretions.Plasma I-FABP levels were significantly increased in TGW (median: 2,199 pg/mL) compared with MSM (median: 884 pg/mL, P = 0.02) and CW (median: 681 pg/mL, P = 0.001).In rectal secretions I-FABP levels were also increased in TGW compared with CW (P = 0.01); however, there was no difference observed between TGW and MSM (Figure 6, A and B).Out of the inflammatory biomarkers tested, we found that plasma levels of IL-1RA and MCP-1 were increased in TGW compared with CW (P = 0.008 and P = 0.03, respectively) while only MCP-1 levels were increased in TGW compared with MSM (P = 0.02, Figure 6, C and D).No difference between MSM and CW was observed.Increased plasma levels of IL-1RA and MCP-1 have been previously linked to an increased risk of vaginal HIV acquisition (25,40).There were no differences observed between plasma Zonulin and sCD14 between groups.Several studies have shown that the microbiome composition can impact local as well as systemic inflammation (41).We assessed the rectal microbiome by 16s rRNA in TGW (n = 9), MSM (n = 9), and CW (n = 7), and no differences at the phylum level were observed by Shannon Diversity Index, with a median index of 4.0, 3.8, and 4.1, respectively (data not shown).However, we observed less than 5% abundance of Actinobacteria in the rectal microbiome of all TGW compared with CW and MSM (Figure 6E).We also observed greater than 5% abundance of Fusobacteria in 7/9 TGW participants compared to 3/7 CW and 4/9 MSM.The abundance of Actinobacteria inversely correlated with plasma I-FABP levels (r = -0.46,P = 0.03; Figure 6F), while the abundance of Fusobacteria directly correlated with rectal I-FABP (r=0.39,P = 0.05; Figure 6G) and plasma MCP-1 (r = 0.44, P = 0.04; Figure 6H) levels, indicative of differing rectal microbiome composition in TGW compared with MSM and CW.This may disrupt mucosal homeostasis, as indicated by the increased enterocyte damage and an increased rectal inflammation evidenced by elevated MCP-1 levels.

Discussion
TGW are among the most at-risk populations for HIV acquisition globally, which is still mainly attributed to increased sociodemographic and behavioral risk factors exacerbated by lack of gender-affirming care, decreased access to health care, and gender-based discrimination (42,43).The main aim of the current study was to evaluate potential underlying biological risks of HIV acquisition in TGW, an important  and E).In addition, a direct correlation between the Shannon Diversity Index and the cycling of Ki67-expressing CD4 + T cells was observed (F).Differences between groups were analyzed using unpaired t tests.Spearman's correlation was used to analyze associations between 2 variables.Brown circles, vaginal; purple squares, neovaginal.
step toward further tailoring HIV prevention and treatment programs to this key population (44).To our knowledge, this study provides the first in-depth insight into a potential biological mechanism that could contribute to the higher risk of HIV acquisition in TGW based on the presence of CD4 + CCR5 + target cells on the neovaginal surface reconstructed by penile skin inversion and the increased frequency of those target cells in the rectal mucosa.
We observed CD3 + T cells in the penile skin-lined neovagina, which is not too surprising, since human skin, as the largest and primary interface with the environment, contains large numbers of CD3 + T cells, with some of them residing in the epidermis (45,46).In addition, several studies have shown that the inner and/or outer foreskin, potentially included in the reconstruction of the neovagina, is rich in CD3 + T cells (47,48), thus posing an additional source of CD3 + T cells on neovaginal surfaces after GAS by penile skin inversion.However, the composition of CD3 + T cell subsets was vastly different on the neovaginal surface compared with the vaginal surface, showing a higher frequency of CD8 + and lower frequency of CD4 + T cells, with both subsets having increased metabolic activity and cell proliferation indicated by the expression of Ki67.Previous studies have shown that increased expression of Ki67 on cervical CD4 + T cells was linked to higher HIV susceptibility (27); thus, our results could indicate a greater risk of HIV acquisition in TGW engaging in neovaginal intercourse.The increased expression of PD-1 on CD8 + T cells could be indicative of further immune activation leading to immune exhaustion, which has been linked to an impaired response of mucosal PD-1 + CD8 + T cells, e.g., to Chlamydia trachomatis (49).Additionally, neovaginal levels of the proinflammatory soluble activation markers CRP and sCD30 (50,51) were increased compared with vaginal secretion levels, contributing to an overall proinflammatory neovaginal environment potentially further increasing HIV susceptibility.In CW the vaginal microbiome itself is an important determinant of the levels of local inflammation (25,52,53).Increased vaginal inflammation has been linked to bacterial vaginosis (BV) (54,55), lowering the barrier for HIV infection, and this is in concordance with our observations in the neovaginal compartment showing a polymicrobial microbiome and an enrichment in Prevotella and Streptococcus, all associated with BV in CW (56).Those polymicrobial neovaginal communities were linked to increased inflammation in the neovaginal compartment.This observation is further corroborated by a recent study identifying neovaginas with diverse polymicrobial communities that elicit similar inflammation and host responses observed in BV in CW (24).In addition, we also observed the abundance of Finegoldia magna in the neovaginal compartment, which was previously described as part of the microflora of the penile skinlined neovagina (35) and is otherwise known to colonize skin and nonsterile body surfaces.Finegoldia magna is known to activate neutrophiles through its soluble proteins FAF and L and induces a proinflammatory response (34), thus potentially contributing the a proinflammatory environment in the neovagina.
The abundance of CD4 + CCR5 + target cells in the rectal mucosa serves as another major portal of entry for HIV (57,58).Several studies demonstrated an increased risk of HIV acquisition and other STIs such as gonorrhea and chlamydia in MSM engaging in condomless anal intercourse (59,60).Both nonhuman primate and human studies have linked this increased risk to the specific mucosal CD4 + T cells subsets highly susceptible to HIV infection, such as CD4 + CCR5 + T cells and α 4 β 7 hi memory CD4 + T cells and their activation status (61)(62)(63).In the current study, frequency and absolute cell count of CD4 + CCR5 + T cells in the gut mucosa of TGW were significantly increased compared with MSM.Those findings were corroborated by the observation that the DNA methylation levels related to the CCR5 gene in the gut were decreased in TGW, indicating increased gene expression (39,64,65).Furthermore, the increase in the

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JCI Insight 2023;8(16):e169272 https://doi.org/10.1172/jci.insight.169272frequency of mucosal CD4 + CCR5 + T cells was directly linked to the lack of testosterone after GAS and is potentially also impacted by the usage of supraphysiologic doses of exogenous feminizing hormones (5).A similar association is known in the context of depo medroxyprogesterone hormonal contraception usage in enhancing risk of HIV acquisition in CW (66).Progesterone leads to thinning of the vaginal epithelium, and inhibiting secretion of cytokines and chemokines by innate and adaptive immune cells (11), while progestin-only contraceptives increase the expression of the CCR5 on CD4 + T cells in peripheral blood and vaginal mucosa (7,9,12).Given that TGW in this study reported engaging in both neovaginal and receptive anal intercourse, changes in both compartments are relevant to HIV transmission risk.
In addition to frequency of target cells, 2 critical determinants of the risk for HIV acquisition when engaging in anal intercourse are gut immune activation and the microbiota (67,68).A normal gut microbiota is essential for immune homeostasis.Disruption in intestinal immunity can precipitate gut dysbiosis, which in turn leads to a disruption of the mucosal barrier, microbial translocation, and inflammation in the mucosa and periphery (69)(70)(71)(72).Variations in the gut microbiota can be seen with changes in diet, antimicrobial use, stress, and environmental exposures (73).In addition, several studies have linked changes in the gut and stool microbiome in MSM to sexual preferences, with MSM engaging in receptive anal intercourse having a microbiome enriched for Prevotella species opposed to the microbiota of non-MSM enriched for Bacteroides (74,75).We observed an association between higher abundance of Fusobacteria and lower abundance of Actinobacteria in TGW compared with MSM and CW with enterocyte damage, indicating a disruption of the mucosal homeostasis.Gut-derived Fusobacteria have been identified to have proinflammatory properties and are implicated in human colorectal cancer and chronic gut inflammation such as inflammatory bowel disease and have been associated with suboptimal immune recovery in HIV-infected patients (76)(77)(78).In contrast, Actinobacteria are major phyla of the gut microbiome, and though they represent only a small percentage, are pivotal in maintenance of the gut homeostasis (79).
One major limitation of our study is the modest sample size and the low number of TGW agreeing to undergo voluntary sigmoid biopsy.However, linking the actual frequency of mucosal CD4 + CCR5 + T cells to mucosal DNA methylation status of the CCR5 gene provides additional fidelity.Nevertheless, further studies with a more robust samples size would be required to further support these initial findings.Due to low acceptance of biopsy collection in this TGW population, cells from the vaginal/neovaginal compartment were collected using swabs instead of biopsy.It is known that 2 cervical swabs yield similar numbers of leucocytes compared to 1 biopsy; however, swabs and biopsies were biased toward macrophage and T lymphocytes, respectively (80), which should be considered when interpreting our results.Another caveat is the differing risk behavior of the CW enrolled in this study compared with TGW.While 40% of the TGW reported a history of sex work with an average of 35 sex partners, no CW reported history of sex work, with an average of 3 sex partners.BV leads to increased inflammation and is known to increase the risk of HIV acquisition (25,53), with BV reaching a prevalence in female sex workers up to 70% (81).Therefore, we cannot exclude that CW with a similar risk behavior to the enrolled TGW would have been presented with a different vaginal microbiome composition, more resembling BV, which in turn can impact cellular and soluble markers of inflammation.We did not control for diet or other environmental factors in the microbiome analyses, and unmeasured confounding factors may explain the difference in the enrichment of Fusobacteria and Actinobacteria we are observing in TGW compared with CW and MSM.In addition, the effects of estrogen and progesterone have been studied extensively in the context of oral contraceptives in CW.Progesterone is known to induce thinning of the vaginal epithelium, inhibiting cytokine and chemokine secretion from T cells, macrophages, and dendritic cells and thus dampening the immune response (11).Conversely, estrogen might have a protective effect by decreasing CCR5 expression and inducing interferon-α and entry-mediated mechanisms (82,83).For these reasons, we enrolled only CW not using hormonal contraceptives.However, systematic studies on the effect of exogenous hormones on the neovagina in TGW have not been reported, and our data are not able to account for potential biases such as different feminizing hormone treatments in our TGW cohort due to the small sample size.Finally, our findings are generally descriptive in nature, and further experiments or large studies will be necessary to determine the relevance of those mucosal findings in the neovagina and gut mucosa in TGW regarding their risk of HIV acquisition.
Overall, this study highlights the need to better understand potential biological factors unique to TGW that could influence the risk of HIV acquisition, such as increased inflammation due to GAS or changes pertaining to the continuous usage of supraphysiologic doses of exogenous feminizing hormones.The better understanding of those risks would be an important next step toward more comprehensive health care services tailored to
DNA extraction and PCR amplification.Vaginal, neovaginal, and rectal swabs were collected using FLOQSwabs (Copan Diagnostics) and stored at -80°C.For assessment of the microbiome ZymoBIO-MICS DNA Microprep Kit was utilized for a standardized cellular lysis of swabs and to isolate bacterial DNA for 16s library preparation.The kit utilizes a bead-beat sample, spin-column purification, and filter to remove PCR inhibitors.DNA was quantified utilizing a NanoDrop instrument (Thermo Fisher Scientific), and 50 ng total DNA was input into 16s library construction.
Next-generation sequencing and data processing.For vaginal and neovaginal swabs, the Swift Biosciences 16s+ ITS panel was utilized to generate sequencing libraries.A total of 25 nanograms of DNA was utilized for input into a multiplex PCR targeting all variable regions of the 16s rRNA gene.Dual-indexed Illumina sequencing adapters were utilized to barcode samples.Quantitative PCR was utilized to quantify libraries for pooling.Sequencing was performed on an Illumina iSeq 100 system generating 2 × 150 bp paired-end reads.For rectal swabs, a 2-step PCR protocol targeting the V4 region of the 16s rRNA gene was utilized to generate 16s libraries according to published protocols (86).Sequencing was obtained on an Illumina MiSeq system generating 2 × 300 bp paired-end reads.The raw sequencing data generated were processed by filtering raw fastq files for primer an adapter dimer sequences, then removing contaminating host sequences, and analyzed using both Qiime 1.9.1 analysis software and the One Codex data platform.Sequencing data were uploaded to the NIH NCBI GEO database (accession number GSE234969).
Epigenetic DNA methylation profiling of gut tissues.For Illumina EPIC Array-Based DNA Methylation Analysis, 500 ng of DNA per sample were isolated from biopsies lysed in RLT buffer using the QIA-GEN TissueRuptor II and AllPrep DNA/RNA/miRNA Universal Kit for all types of tissues.DNA was bisulfite-converted using the EZ DNA Methylation kit (Zymo Research) according to the manufacturer's instructions.Bisulfite-converted DNA samples were randomly assigned to a chip well on the Infinium HumanMethylationEPIC BeadChip, amplified, hybridized onto the array, stained, washed, and imaged with the Illumina iScan SQ instrument to obtain raw image intensities.Raw Methylation EPIC array IDAT intensity data were loaded and preprocessed in the R statistical programming language (http://www.r-project.org)using the Chip Analysis Methylation Pipeline (ChAMP, version 2.8.3).IDAT files were loaded using the champ.loadfunction.All samples passed quality control metrics.Comprehensive filtering was applied to the data set for probes with detection P values < 0.01, all non-CpG probes, previously published SNP-related probes, multihit probes, and probes on sex chromosomes.Methylation β-values ranging 0-1 (corresponding to unmethylated to methylated signal intensity) for each sample were normalized using the BMIQ function implemented in the ChAMP pipeline.DNA methylation loci related to the CCR5 gene were annotated using the EPIC array R package annotation IlluminaHumanMethylationEPICanno.ilm10b4.hg19.Methylation β-values were utilized and represent a quantification at each CpG site of β = M/(M + U + a), where M > 0 and U > 0 denote the methylated and unmethylated signal intensities.
Statistics.All statistical analysis was performed using Prism version 8.2.0 for Mac OS (Graph-Pad Software).The paired/unpaired t test was used for between group comparisons, while Kruskal-Wallis was used for comparison between more than 2 groups.Spearman's rank test was used to evaluate associations.All values reported were median (interquartile range).Statistical tests were 2 sided with P ≤ 0.05 considered statistically significant.For multiple comparisons Dunn's test was performed with Benjamini-Hochberg multiple comparisons adjustment.
Study approval.All studies were approved by the Chulalongkorn University and Walter Reed Army Institute of Research institutional review boards for human research.Written informed consent was obtained from all participants prior to inclusion in the studies.The investigators have adhered to the policies for protection of human participants as prescribed in AR-70-24 (Use of volunteers as subjects of research, US Army Regulation: Research and Development).

Figure 1 .
Figure 1.Frequency of CD4 + and CD8 + T cells differs between the vagina and the neovagina.(A) Example of parent gating strategy of freshly isolated neovaginal mononuclear cells.The staining profile of vaginal mononuclear cells from a representative volunteer is shown, indicating the frequency of activated CD4 + and CD8 + T cells by the expression of Ki67 and coexpression of CD38 and HLA-DR.(B) Frequency of vaginal (n = 8) and neovaginal (n = 7) CD4 + T cells and (C) CD8 + T cells, (D) CD4/CD8 T cell ratio, and (E) frequency of CD4 + CCR5 + T cells are shown depending on sample availability.Difference between groups were analyzed using unpaired t test.

Figure 2 .
Figure 2. Cellular and soluble inflammation profiles differ between the vagina and the neovagina.Frequencies of cycling CD4 + (A) and CD8 + (E) T cells are increased in the neovagina (n = 7) compared with the vagina (n = 8), while there is no difference in the frequency of cycling CD4 + CCR5 + (C) T cells observed between the vagina and the neovagina.In contrast, CD4 + (B), CD4 + CCR5 + (D), and CD8 + (F) T cells in the vagina had a higher activation status compared with the neovagina indicated by the coexpression of HLA-DR and CD38.However, there was also a significant increase in PD-1-expressing CD8 + T cells observed in the neovagina (G), which was not seen in CD4 + T cells (data not shown).The only soluble biomarkers out of 18 tested that were significantly different between the vagina and the neovagina were the inflammation makers CRP (median: neovaginal 548 pg/mL vs. vaginal 480 pg/mL) (H) and sCD30 (median: neovaginal 2.45 pg/mL vs. vaginal 0.55 pg/mL) (I) that were significantly increased in neovaginal secretions (H).Differences between groups were analyzed using unpaired t tests.

Figure 3 .
Figure 3. Microbial profiles determined by 16s rRNA sequencing revealed distinct microbial community structures between the vagina and neovagina.(A) Bar graph of phylum rank of vaginal microbiome in cisgender women (CW; n = 10) and neovaginal microbiome in transgender women (TGW; n = 10), including Shannon Alpha Diversity displayed below bar graphs, suggesting a different microbiome composition between the vaginal and the neovaginal compartment.(B) Neovaginal samples have a unique microbiome profile compared with vaginal samples based on principal component analyses.Brown circles, vaginal; purple circles, neovaginal.(C) Heatmap of relative abundance plot of species in neovaginal/vaginal compartment highlighting differences in compositions between vaginal and neovaginal microbiome.

Figure 4 .
Figure 4. Distinct microbial community structures in the neovagina correlate with markers of local inflammation.Neovaginal (n = 10) microbial communities are characterized by a higher Shannon Diversity Index (A), a higher Prevotella abundance (B), and a lower Lactobacillus abundance (C) compared with vaginal (n = 10) microbial communities.The higher Shannon Diversity Index in the neovagina was linked to an increased inflammation profile, indicated by elevated neovaginal CRP and sCD30 levels (Dand E).In addition, a direct correlation between the Shannon Diversity Index and the cycling of Ki67-expressing CD4 + T cells was observed (F).Differences between groups were analyzed using unpaired t tests.Spearman's correlation was used to analyze associations between 2 variables.Brown circles, vaginal; purple squares, neovaginal.

Figure 5 .
Figure 5. Frequency of CD4 + CCR5 + T cells in the sigmoid colon in MSM (n = 10), CW (n = 9), and TGW (n = 3).The frequency (A) and absolute number (B) of colonic CD4 + CCR5 + T cells was higher in CW and TGW compared with MSM.(C) Altered DNA methylation at loci cg22066626 at regulatory region of CCR5 gene in the gut mucosa in TGW compared with CW and MSM.(D) DNA methylation levels related to CCR5 gene were inversely associated with the frequency of gut mucosa CD4 + CCR5 + T cells.(E) The frequency of colonic CD4 + CCR5 + T cells was indirectly correlated with the plasma testosterone levels.Differences between groups were adjusted for multiple comparisons using Dunn's test with Benjamini-Hochberg multiple comparison adjustment.Spearman correlation was used to analyze association between 2 variables.Orange triangles, MSM; brown triangles, CW; purple triangles, TGW.

Figure 6 .
Figure 6.Differences in levels of soluble biomarkers in plasma/rectal secretions and rectal microbial profiles in MSM (plasma/rectal n = 9), CW (plasma n = 9, rectal n = 7), and TGW (plasma n = 9, rectal n = 10).The levels of I-FABP in (A) plasma and in rectal secretions (B) were increased in TGW compared with MSM and/or CW, indicating increased enterocyte damage.In addition, an increase in plasma IL-1RA (C) and MCP-1 (D) was observed in TGW compared with MSM and/or CW. (E) Bar graph of phylum rank of rectal microbiome in MSM (n = 9), CW (n = 7), and TGW (n = 9), including Shannon Diversity Index, suggesting differences in the level of Fusobacteria and Actinobacteria, with the abundance of Actinobacteria inversely correlating with plasma I-FABP levels (F) and the abundance of Fusobacteria directly correlating with rectal I-FABP (G) and plasma MCP-1 levels (H).Spearman correlation was used to analyze associations between 2 variables.Orange triangles, MSM; brown triangles, CW; purple triangles, TGW.